Journal: iScience

Article Title: Prenatal bisphenol A exposure reprograms SF1-lactylation pathways to promote endometriosis susceptibility

doi: 10.1016/j.isci.2026.115608

Figure Lengend Snippet: BPA exposure induces mitochondrial dysfunction, mitigated by NaOx or AC45594 HESC were treated with CTL or 50 nM BPA for 3 days, followed by 3-day treatment with vehicle, NaOx (20 mM), or AC45594 (5 μM). (A) Intracellular ATP. (B) Mitochondrial DNA copy number. (C) Mitochondrial membrane potential (ΔΨm). (D) Representative TMRE images. (E) Caspase-3 activity. (F) Apoptosis rate. n means number of independent repeats, n = 5. ∗, p < 0.05 vs. CTL(3d)+CTL(3d)/VEH; ¶, p < 0.05 vs. BPA(3d) + CTL(3d)/VEH. Data are presented as mean ± SD.

Article Snippet: ApoAlert Caspase-3 Activity Kit , Clontech , Cat# 630216.

Techniques: Membrane, Activity Assay

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: According to the instructions of the Caspase-3 Enzyme Activity Assay Kit (Beyotime, Shanghai, China), cells from each group were lysed for 15 min on ice using lysis buffer.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: According to the instructions of the Caspase-3 Enzyme Activity Assay Kit (Beyotime, Shanghai, China), cells from each group were lysed for 15 min on ice using lysis buffer.

Techniques: Activity Assay

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: Effect of Et MIC2 on E.tenella infection rate and Caspase 3 Activity through ITGAV.

Article Snippet: According to the instructions of the Caspase-3 Enzyme Activity Assay Kit (Beyotime, Shanghai, China), cells from each group were lysed for 15 min on ice using lysis buffer.

Techniques: Infection, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: EZH2 Regulates the Proliferation-Senescence Balance and Tumor–Stromal Signaling in Lung Adenocarcinoma

doi: 10.3390/ijms27135914

Figure Lengend Snippet: EZH2 targeting induces oxidative stress without activating apoptotic pathways. ( A ) Relative mRNA expression levels of BAX, BCL2, and BAX/BCL2 ratio determined by RT-qPCR in A549 cells. Data are normalized to control and presented as mean ± SEM. Statistical significance was assessed relative to control (* p < 0.05, ** p < 0.01, *** p < 0.001). ( B ) Caspase-3 activity assay in A549 cells following treatment, with staurosporine as a positive control. Data are presented as percentage of positive control (mean ± SEM). ( C ) DCFH-DA assay measuring intracellular ROS levels in A549 cells following treatment with EPZ6438 and MS1943. Data are presented as percentage of control (mean ± SEM). Statistical significance was assessed relative to control (* p < 0.05, *** p < 0.001).

Article Snippet: Caspase-3 activity was assessed using the Caspase-3 Activity Assay Kit (MedChemExpress, Monmouth Junction, NJ, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Quantitative RT-PCR, Control, Caspase-3 Activity Assay, Positive Control, DCFH-DA Assay

Journal: Bioactive Materials

Article Title: Degradation behavior and biological properties of a novel biodegradable zinc alloy biliary stent

doi: 10.1016/j.bioactmat.2025.12.022

Figure Lengend Snippet: The content of Caspase-3 and ROS in cells treated with Zn alloy extract. A–B: The content of Caspase-3 ( A ) ROS ( B ) and in L929 cells treated with different Zn alloy extract. C-D: The content of Caspase-3 ( C ) ROS ( D ) and in RAW264.7 cells treated with Zn alloy extract. Magnification: 40 × , Scale bar: 10 μm.

Article Snippet: Reactive Oxygen Species Assay Kit and GreenNucTM Live Cell Caspase-3 Activity Assay Kit were purchased from Beyotime (China).

Techniques: